DNMT3B antibody (N-Term)

Catalog No. ABIN6287502

Product Details anti-DNMT3B Antibody

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Binding Specificity: AA 1-80, N-Term

Reactivity: Human, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Dnmt3b is a protein encoded by the DNMT3B gene which is approximately 95,7 kDa. Dnmt3b is localised to the nucleus. It is involved in the mesodermal commitment pathway and sulfur amino acid metabolism. It is a methyltransferase which is thought to function in genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. Dnmt3b is expressed ubiquitously with the highest expression in foetal liver, heart, kidney and placenta. Mutations in the DNMT3B gene may result in immunodeficiency-centromeric. STJ92753 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Dnmt3b protein.

Specificity: Dnmt3b Polyclonal Antibody detects endogenous levels of Dnmt3b protein.

Characteristics: Rabbit polyclonal to Dnmt3b.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Dnmt3b

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Comment:

Ubiquitous, highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: Dnmt3b (DNMT3B Products)

Synonyms: DNMT3B antibody, LOC100218113 antibody, ICF antibody, ICF1 antibody, M.HsaIIIB antibody, MmuIIIB antibody, cb91 antibody, dnmt3bl antibody, dnmt7 antibody, sb:cb91 antibody, wu:fb16h07 antibody, DNA methyltransferase 3 beta antibody, DNA methyltransferase 3B antibody, DNA (cytosine-5-)-methyltransferase 3 beta, duplicate a antibody, DNMT3B antibody, Dnmt3b antibody, dnmt3b antibody, dnmt3ba antibody

Molecular Weight: 96 kDa

Gene ID: 1789

UniProt: Q9UBC3