YWHAB/YWHAZ antibody (pSer184, pSer186)

Catalog No. ABIN6287849

Product Details anti-YWHAB/YWHAZ Antibody

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Binding Specificity: AA 120-200, pSer184, pSer186

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB/YWHAZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: 14-3-3 β/ζ is a protein encoded by the YWHAZ gene which is approximately 27,7 kDa. 14-3-3 β/ζ is localised to the cytoplasm and melanosome. It is involved in the activation of cAMP-dependent PKA, development slit-robo signalling and activation of BH3-only proteins. It is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signalling pathways. It binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. 14-3-3 β/ζ is expressed in the nervous system, intestine, skin, blood and eye. STJ90562 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous 14-3-3 β/ζ protein which only binds about S184/186 when S184/186 is phosphorylated.

Specificity: Phospho-14-3-3 β/ζ (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein only when phosphorylated at S184/186.

Characteristics: Rabbit polyclonal to Phospho-14-3-3 β/ζ (S184/186).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of S184/186.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ELISA 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for YWHAB/YWHAZ

Target: YWHAB/YWHAZ (14-3-3 beta/zeta (YWHAB/YWHAZ))

Alternative Name: 14-3-3 beta/zeta (YWHAB/YWHAZ Products)

Synonyms: 14-3-3z antibody, 14-3-3zeta antibody, kcip-1 antibody, ywhaq antibody, Ywhaz antibody, ACYPI003154 antibody, 1433z antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta antibody, 14-3-3 protein zeta antibody, ywhaz antibody, 14-3-3zeta antibody, YWHAZ antibody, 1433z antibody

Molecular Weight: 28 kDa

Gene ID: 7534

UniProt: P63104