KIT antibody (AA 100-300)

Catalog No. ABIN6294267

The Mouse Monoclonal anti-KIT antibody (ABIN6294267) specifically detects KIT in WB, FACS, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for KIT antibody (AA 100-300) (ABIN6294267)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-KIT Antibody

Binding Specificity: AA 100-300

Purpose: Mouse anti-Human c-Kit Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.

Immunogen: A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: (0.5-1 μg/mL for 2 hours at RT)
Immunohistochemistry (Frozen Only): 2-4 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: Mast/stem cell growth factor receptor Kit

Background:

Target Description: This mAb recognizes a protein of 145 kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Gene Symbol: KIT///SCFR

Gene ID: 3815

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity