TNF alpha antibody (AA 115-130)

Catalog No. ABIN6294276

The Mouse Monoclonal anti-TNF alpha antibody (ABIN6294276) specifically detects TNF alpha in IHC, FACS and IF. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio) and Cat samples.

Quick Overview for TNF alpha antibody (AA 115-130) (ABIN6294276)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio), Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 115-130

Purpose: Mouse anti-Human/Mouse/Rat/Rabbit/Cat/Dog/Zebrafish TNF alpha Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and extracellular (secreted)

Purification: A hexadecapeptide corresponding to aa 115-130 of human TNF alpha, conjugated to thyroglobulin, was used as the immunogen for this TNF alpha antibody.

Immunogen: A hexadecapeptide corresponding to aa 115-130 of human TNF alpha, conjugated to thyroglobulin, was used as the immunogen for this TNF alpha antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor necrosis factor , C-domain 1, C-domain 2, Tumor necrosis factor, soluble form]

Background:

Target Description: This antibody is specific for a 17-26 kDa protein, which is identified as the cytokine TNF alpha (Tumor Necrosis Factor alpha). It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. It is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. It exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Gene Symbol: TNF///TNFA///TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome