BAX antibody (AA 3-16)

Catalog No. ABIN6294454

This Mouse Monoclonal antibody specifically detects BAX in WB, IHC, IF and FACS. It exhibits reactivity toward Human and Monkey.

Quick Overview for BAX antibody (AA 3-16) (ABIN6294454)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-BAX Antibody

Binding Specificity: AA 3-16

Purpose: Mouse anti-Human/Monkey Bax Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

Immunogen: Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Apoptosis regulator BAX

Background:

Target Description: Recognizes a protein of 21 kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Gene Symbol: BAX///BCL2L4

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response