Bcl-2 antibody (AA 41-54)

Catalog No. ABIN6294520

This Mouse Monoclonal antibody specifically detects Bcl-2 in WB, IHC, IF, FACS, ELISA and Coat. It exhibits reactivity toward Human.

Quick Overview for Bcl-2 antibody (AA 41-54) (ABIN6294520)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 41-54

Purpose: Mouse anti-Human Bcl-2 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, membrane

Purification: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Application Details

Application Notes: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Apoptosis regulator Bcl-2

Background:

Target Description: This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Gene Symbol: BCL2

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling