Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN6295487

The Mouse Monoclonal anti-Smooth Muscle Actin antibody is suitable to detect Smooth Muscle Actin in samples from Human, Rat, Mouse, Chicken, Rabbit, Cow, Monkey, Guinea Pig, Baboon, Pig, Dog, Sheep, Goat and Cat. It has been validated for WB, IF, FACS and IHC (fp).

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN6295487)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Cow, Monkey, Guinea Pig, Baboon, Pig, Dog, Sheep, Goat, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Human/Baboon/Monkey/Cow/Pig/Sheep/Goat/Cat/Dog/Rabbit/Mouse/Rat/Guinea pig/Chicken. Smooth Muscle Actin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.

Immunogen: An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.

Application Details

Application Notes: Western blot: 0.5-1 μg/mL
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: Actin, aortic smooth muscle

Background:

Target Description: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.

Gene Symbol: ACTA2///ACTSA///ACTVS///GIG46

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development