KRT8 antibody

Catalog No. ABIN6295957

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Cow, Pig, Sheep and Monkey.

Quick Overview for KRT8 antibody (ABIN6295957)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Cow, Pig, Sheep, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-KRT8 Antibody

Purpose: Mouse anti-Human/Monkey/Cow/Sheep/Pig Cytokeratin 8 + 18 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 + 18 antibody.

Immunogen: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 + 18 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Keratin, type II cytoskeletal 8

Background:

Target Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Gene Symbol: KRT8///CYK8///KRT18///CYK18///PIG46

UniProt: P05787, P05783