XRCC6 antibody

Catalog No. ABIN6296746

This Mouse Monoclonal antibody specifically detects XRCC6 in IF, FACS and ICC. It exhibits reactivity toward Human and Primate.

Quick Overview for XRCC6 antibody (ABIN6296746)

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-XRCC6 Antibody

Purpose: Mouse anti-Human/Primates Ku70 + Ku80 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Application Details

Application Notes: Flow cytometry: 0.5-1 μg/10e6 cells
IF: 0.5-1.0 μg/mL
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: X-ray repair cross-complementing protein 6

Background:

Target Description: This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Gene Symbol: XRCC6///G22P1

Gene ID: 2547

UniProt: P12956

Pathways: DNA Damage Repair