PCNA antibody

Catalog No. ABIN6296907

The Mouse Monoclonal anti-PCNA antibody (ABIN6296907) specifically detects PCNA in WB, IHC, FACS and IF. The antibody is reactive with Human, Mouse, Rat, Chicken, Pig, Monkey, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Drosophila melanogaster and Schizosaccharomyces pombe samples.

Quick Overview for PCNA antibody (ABIN6296907)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Pig, Monkey, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-PCNA Antibody

Purpose: Mouse anti-Human/Mouse/Rat/Monkey/Pig/Chicken/Zebrafish/Drosophila melanogaster/Yeast PCNA Antibody [Sodium Azide Free]

Specificity: Predominantly nuclear, some cytoplasmic

Purification: Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Immunogen: Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: Proliferating cell nuclear antigen

Background:

Target Description: This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Gene Symbol: PCNA

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy