GFM1 antibody (G-Elongation Factor, Mitochondrial 1)

Details for Product anti-GFM1 Antibody No. ABIN6297765
Antigen
  • COXPD1
  • EFG
  • EFG1
  • EFGM
  • EGF1
  • GFM
  • hEFG1
  • AW545374
  • D3Wsu133e
  • Gfm
  • EF-G
  • Efg
  • G elongation factor mitochondrial 1
  • G elongation factor, mitochondrial 1
  • GFM1
  • Gfm1
Reactivity
Human
68
32
30
4
3
2
2
2
2
2
1
1
Host
Mouse
53
17
Clonality
Monoclonal
Conjugate
This GFM1 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
52
21
21
14
13
12
4
3
2
2
1
Options
Purpose Mouse anti-Human Mitochondrial Antibody [Sodium Azide Free]
Immunogen The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
Specificity Mitochondria in cytoplasm
Purification The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
Plasmids, Primers & others Plasmids, Primers & others GFM1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Mitochondrial (GFM1 Antibody Abstract)
Background

Target Description: MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Application Notes FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.25-0.5 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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