GFM1 antibody

Catalog No. ABIN6297765

Product Details anti-GFM1 Antibody

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse anti-Human Mitochondrial Antibody [Sodium Azide Free]

Specificity: Mitochondria in cytoplasm

Purification: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.

Immunogen: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.25-0.5 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: Mitochondrial (GFM1 Products)

Synonyms: COXPD1 antibody, EFG antibody, EFG1 antibody, EFGM antibody, EGF1 antibody, GFM antibody, hEFG1 antibody, AW545374 antibody, D3Wsu133e antibody, Gfm antibody, EF-G antibody, Efg antibody, G elongation factor mitochondrial 1 antibody, G elongation factor, mitochondrial 1 antibody, GFM1 antibody, Gfm1 antibody

Background:

Target Description: MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.