TNF alpha antibody (Biotin)

Catalog No. ABIN6297805

This Mouse Monoclonal antibody specifically detects TNF alpha in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TNF alpha antibody (Biotin) (ABIN6297805)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 5H4-F10-E11

Product Details anti-TNF alpha Antibody (Biotin)

Purpose: Mouse anti-Human TNF alpha Biotinylated Antibody

Immunogen: This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.

Isotype: IgG3

Application Details

Application Notes: ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000)

Restrictions: For Research Use only

Handling

Reconstitution: A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e. g. sodium azide). The final amount is indicated on the shipping vial.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: -20°C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background:

Target Description: TNF alpha is an  adipokine  involved in systemic  inflammation  and is a member of a group of cytokines that stimulate the  acute phase reaction. It is produced chiefly by activated  macrophages  (M1), although it can be produced by many other cell types such asCD4+ lymphocytes,  NK cells, neutrophils, mast cells, eosinophils, and neurons.

UniProt: P78536

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome