CHFR antibody (N-Term)
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- Target See all CHFR Antibodies
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CHFR antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- CHFR antibody was raised against the N terminal of CHFR
- Purification
- Affinity purified
- Immunogen
- CHFR antibody was raised using the N terminal of CHFR corresponding to a region with amino acids REWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVIN
- Top Product
- Discover our top product CHFR Primary Antibody
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- Application Notes
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WB: 1 µg/mL
Optimal conditions should be determined by the investigator. - Comment
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CHFR Blocking Peptide, catalog no. 33R-7895, is also available for use as a blocking control in assays to test for specificity of this CHFR antibody
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of CHFR antibody in PBS
- Concentration
- Lot specific
- Buffer
- PBS
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- Target
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
- Alternative Name
- CHFR (CHFR Products)
- Synonyms
- RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody
- Background
- CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
- Molecular Weight
- 69 kDa (MW of target protein)
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