COL6 antibody (Biotin)

Catalog No. ABIN636758

Product Details anti-COL6 Antibody (Biotin)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Characteristics: Rabbit polyclonal Collagen Type VI antibody (biotin) conjugated
Bioactivity: Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Purification: Affinity chromatography purified

Immunogen: Collagen type VI antibody was raised in rabbit using collagen type VI from human and bovine placenta as the immunogen.

Application Details

Application Notes: ELISA: 1:10,000-1:50,000, IHC: 1:200-1:1,000, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 0.125 M Na5 5 7, pH 8.0, 0.075 NaCl, 0.005 EDTA, 10 mg/mL BSA, and 0.01 % NaN3. Immunoglobulin and protease free.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Abstract: COL6 Products

Background: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Collagen alpha-1(VI) chain is a protein that in humans is encoded by the COL6A1 gene. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively.