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Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Alkaline Phosphatase (AP)
Catalog No. ABIN637625
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2356
    • 501
    • 411
    • 188
    • 130
    • 124
    • 94
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 697
    • 678
    • 568
    • 452
    • 368
    • 239
    • 200
    • 148
    • 140
    • 132
    • 122
    • 98
    • 96
    • 95
    • 94
    • 63
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 1728
    • 1485
    • 537
    • 215
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Donkey
    Conjugate
    • 586
    • 410
    • 408
    • 369
    • 298
    • 276
    • 228
    • 120
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Alkaline Phosphatase (AP)
    Specificity
    This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
    Cross-Reactivity (Details)
    No reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, Goat, Guinea Pig, hamster, horse, human, mouse, rabbit or sheep.
    Characteristics
    Donkey anti Rat IgG (H + L) secondary antibody (Alk Phos)
    Alternative Names: Donkey anti Rat Immunoglobulin G (H + L) secondary antibody (Alk Phos)
    Physical state: Clear, colorless liquid
    Purification
    Affinity purified using solid phase antibodies
    Purity
    > 95 % pure
    Immunogen
    Purified Rat IgG (H&L)
  • Application Notes
    1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC
    Comment

    Country of Origin: Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA and 0.05 % (w/v) Sodium Azide. Protease/IgG free, pH 7.2.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted liquid at 2-8 °C. For storage at -20 °C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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