IGF1 antibody (Biotin)

Catalog No. ABIN640174

This Rabbit Polyclonal antibody specifically detects IGF1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IGF1 antibody (Biotin) (ABIN640174)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IGF1 Antibody (Biotin)

Purpose: Rabbit Anti-Human IGF1 [+Biotin]

Specificity: DS-PB-02646 is specific for human IGF-I (Insulin-like Growth Factor I), a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumor cell growth. IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signaling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signaling pathway.

Characteristics: Cytokines/Chemokines/Growth Factors/Receptors

Immunogen: Antisera to human IGF-I were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Isotype: IgG

Application Details

Application Notes: ELISA (dilution: 0.1, 0.2 μg/mL), Western Blotting

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 0.5 mL sterile PBS containing 0.1 % Bovine Serum Albumin.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % sodium azide is recommended.

Concentration: IgG concentration 0.1 mg/ml

Buffer: Phosphate buffered saline pH7.2

Storage: -20 °C

Storage Comment: -20°C

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-Like Growth Factor I

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation