RLTPR antibody (PE)
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- Target See all RLTPR products
- RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))
- Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This RLTPR antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
- Cross-Reactivity (Details)
- Human, Mouse
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Murine RLTPR
- Clone
- EM-53
- Isotype
- IgG1 kappa
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- Application Notes
- Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
- Comment
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))
- Alternative Name
- RLTPR / CARMIL2 (RLTPR Products)
- Synonyms
- CG1399-PB antibody, D130029J02Rik antibody, Gm585 antibody, CARMIL2 antibody, CARMIL2b antibody, LRRC16C antibody, capping protein regulator and myosin 1 linker 2 antibody, F-actin-uncapping protein RLTPR antibody, RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing antibody, Carmil2 antibody, CARMIL2 antibody, LOC100021093 antibody, LOC100091608 antibody, RLTPR antibody
- Background
- Capping protein regulator and myosin 1 linker 2,RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing, capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 Molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.,CARMIL2, LRRC16C
- Gene ID
- 146206
- UniProt
- Q6F5E8
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