Grancalcin antibody (AA 42-217)

Catalog No. ABIN6654161

Product Details anti-Grancalcin Antibody

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Binding Specificity: AA 42-217

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Grancalcin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antigen affinity purified

Immunogen: Amino acids A42-I217 from the human protein were used as the immunogen for the Grancalcin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Grancalcin antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Direct ELISA: 0.1-0.5 μg/mL (human recombinant protein)

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Grancalcin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Grancalcin

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Alternative Name: Grancalcin / GCA (GCA Products)

Synonyms: Grancalcin antibody, 5133401E04Rik antibody, AI573844 antibody, GCL antibody, zgc:92027 antibody, grancalcin antibody, Grancalcin antibody, zgc:92027 antibody, GCA antibody, gran antibody, LOC100280890 antibody, Gca antibody

Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction, with magnesium alone, it partitions with the granule fraction, and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

UniProt: P28676