Recombinant NGFR antibody (AA 281-421)

Catalog No. ABIN6654289

The Rabbit Monoclonal anti-NGFR antibody is suitable to detect NGFR in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Recombinant NGFR antibody (AA 281-421) (ABIN6654289)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NGFR-2550R

Product Details anti-NGFR Antibody

Binding Specificity: AA 281-421

Purpose: Recombinant NGFR Antibody / p75NTR

Purification: Protein A affinity chromatography

Immunogen: A portion of amino acids 281-421 from the human protein was used as the immunogen for the recombinant NGFR antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant NGFR antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background: This antibody is specific for a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or p75 Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR/p75NTR is a reliable marker for desmoplastic and neurotropic melanomas. It is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR/p75NTR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding