ABCA4 antibody

Catalog No. ABIN6655047

Supplier Product No.: 200-301-d05

This Mouse Monoclonal antibody specifically detects ABCA4 in WB, IHC and ELISA. It exhibits reactivity toward Cowand has been mentioned in 1 publication.

Quick Overview for ABCA4 antibody (ABIN6655047)

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3F4

Product Details anti-ABCA4 Antibody

Supplier Product No.: 200-301-d05

Supplier: Rockland

Purpose: ABCA4 Rim Protein Antibody

Cross-Reactivity (Details): Anti-ABCA4 antibody is directed against bovine ABCA4 Rim Protein.

Purification: The antibody was Protein G purified from culture supernatant.

Immunogen: Anti-ABCA4 antibody was produced by repeated immunizations with 220-kDa (full length) partially purified native bovine disc rim protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: IF
Anti-ABCA4 antibody has been tested by western blot and IHC and is suitable for use in ELISA. Specific conditions should be optimized by the end user. Expect a band of ~220kDa in size corresponding to ABCA4 proteins in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ABCA4 antibody (ABIN6655047)

Cowan, Renner, De Gennaro, Gross-Scherf, Goldblum, Hou, Munz, Rodrigues, Krol, Szikra, Cuttat, Waldt, Papasaikas, Diggelmann, Patino-Alvarez, Galliker, Spirig, Pavlinic, Gerber-Hollbach, Schuierer, Srdanovic, Balogh, Panero, Kusnyerik, Szabo, Stadler, Org: "Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution." in: Cell, Vol. 182, Issue 6, pp. 1623-1640.e34, (2021) (PubMed).

Target Details for ABCA4

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Alternative Name: ABCA4 Rim Protein

Background:

Synonyms: Retinal-specific ATP-binding cassette transporter, ATP-binding cassette sub-family A member 4, RIM ABC transporter

Background: ABCA4 antibody detects ABCA4 (ATP-binding cassette, sub-family A (ABC1), member 4, Rim Protein) a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABCA4 is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essential molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease and are associated with retinitis pigmentosa-19 and age-related macular degeneration. Defects in ABCA4 are the cause of Stargardt disease type 1 (STGD1). STGD is one of the most frequent causes of macular degeneration in childhood. Defects in ABCA4 are also known to cause fundus flavimaculatus (FFM), age-related macular degeneration type 2 (ARMD2) and cone-rod dystrophy type 3 (CORD3). Anti-ABCA4 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: ABCA4

Gene ID: 11304

NCBI Accession: NP_776646

UniProt: O35600