TBP antibody (N-Term)

Catalog No. ABIN6655224

Product Details anti-TBP Antibody

Target: TBP (TATA Box Binding Protein (TBP))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-TBP Antibody was purified by ammonium sulphate precipitation followed by dialysis.

Immunogen:

Immunogen: Anti-TBP Antibody was produced in mice by repeated immunizations containing an amino acid sequence from the N-terminus of human TBP.

Immunogen Type: Peptide

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-TBP Antibody is suitable for Chromatin Immunoprecipitation and Sequencing and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in the appropriate cell lysate or extract.

ChIP Dilution: 4-5 μg per IP

Western Blot Dilution: 1:200 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.02 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP (TBP Products)

Synonyms: GTF2D antibody, GTF2D1 antibody, HDL4 antibody, SCA17 antibody, TFIID antibody, Gtf2d antibody, fd20h06 antibody, si:zc101n13.2 antibody, wu:fd20h06 antibody, zgc:66213 antibody, TBP1 antibody, TBP antibody, tbp antibody, CG9874 antibody, Dmel\\CG9874 antibody, RBP antibody, TBP38 antibody, TFIIDtau antibody, TfIID antibody, dTBP antibody, dTFIID antibody, dTbp antibody, dtbp antibody, gtf2d antibody, gtf2d1 antibody, sca17 antibody, tfiid antibody, tfiidtau antibody, xtbp antibody, tbp-a antibody, tbp-b antibody, TATA-box binding protein antibody, TATA box binding protein antibody, TATA binding protein antibody, TATA-box binding protein L homeolog antibody, TATA-box-binding protein antibody, TATA-box binding protein S homeolog antibody, TBP antibody, Tbp antibody, tbp antibody, tbp.L antibody, tbp.S antibody

Background:

Synonyms: TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, GTF2D1, TF2D, TFIID

Background: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Anti-TBP Antibody is ideal for research in Gene Expression and Transcription.

Gene Name: TBP

Gene ID: 6908

NCBI Accession: NP_001165556

UniProt: P20226

Pathways: WNT Signaling