POLR2A/RPB1 antibody
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- Target See all POLR2A/RPB1 (POLR2A) Antibodies
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This POLR2A/RPB1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Purification
- Anti-Pol II Antibody was purified by Protein A chromatography. Cross-reactivity with Pol II from other sources has not been determined.
- Immunogen
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Immunogen: Anti-Pol II Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II.
Immunogen Type: Peptide
- Isotype
- IgG1
- Top Product
- Discover our top product POLR2A Primary Antibody
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- Application Notes
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Application Note: Anti-Pol II Antibody is suitable for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 225 kDa in the appropriate cell lysate or extract.
ChIP Dilution: 1 μg/ChIP
ELISA Dilution: 1:3,000
Western Blot Dilution: 1:1,000
IF Microscopy Dilution: 1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02 % (w/v) Sodium AzideStabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
- Alternative Name
- Pol II (POLR2A Products)
- Synonyms
- NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody
- Background
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Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2, RNAPII
Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II Antibody is ideal for research in Gene Expression, Transcription and Genetics.
Gene Name: POLR2A
- Gene ID
- 5430
- NCBI Accession
- NP_000928
- UniProt
- P24928
- Pathways
- Regulatory RNA Pathways
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