Amyloid Oligomers antibody

Catalog No. ABIN6655723

Product Details

Target: Amyloid Oligomers

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine)

Purification: Anti-Amyloid Oligomers (A11) Antibody was purified by Protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with Amyloid Oligomers (A11) from Eukaryotes, Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils. Cross-reactivity with Amyloid Oligomers (A11) from other sources has not been determined.

Immunogen:

Immunogen: Amyloid Oligomers (A11) Antibody was produced from whole rabbit serum prepared by repeated immunizations with synthetic molecular mimic of soluble oligomers.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1000-10,000

Application Note: Anti-Amyloid Oligomers (A11) Antibody is suitable for use in IP, IF microscopy, IHC, ELISA, WB, and Dot blot. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: 1:1000

ELISA Dilution: 0.1-10 μg/mL

Western Blot Dilution: 1:1000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References

Peng, Achariyar, Li, Liao, Mestre, Hitomi, Regan, Kasper, Peng, Ding, Benveniste, Nedergaard, Deane: "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." in: Neurobiology of disease, Vol. 93, pp. 215-25, (2018) (PubMed).

Target Details

Target: Amyloid Oligomers

Alternative Name: Amyloid Oligomers (A11)

Background:

Synonyms: Rabbit Anti-Amyloid Oligomer αβ Antibody, Rabbit Anti-Amyloid Oligomer alpha beta Antibody, Rabbit Anti-Amyloid Oligomers A11 Antibody, Amyloid beta A4 protein, Amyloid Oligomer AlphaBeta Antibody, APP Antibody, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, A4, AD1

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease. Anti-Amyloid Oligomers A11 Antibody is useful for researchers interested in Neuroscience research.

Gene Name: APP

Gene ID: 351

NCBI Accession: NM_000484

UniProt: P05067