TJP1 antibody

Catalog No. ABIN6655950

Product Details anti-TJP1 Antibody

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine)

Purification: This affinity purified antibody is directed against human ZO-1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.

Immunogen:

Immunogen: Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100 - 1:200

Application Note: Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1 μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10 μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

ELISA Dilution: 5 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: ZO-1 (TJP1 Products)

Synonyms: ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody

Background:

Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

Gene Name: TJP1

Gene ID: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization