TRPC7 antibody (C-Term)

Catalog No. ABIN6656403

Product Details anti-TRPC7 Antibody

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPC7 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-TrpC7 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with TrpC7 from human, mouse, and rat based on 100% homology with the immunizing sequence. Cross-reactivity with TrpC7 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: TrpC7 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to a near c-terminus region of human TrpC7.

Immunogen Type: Peptide

Clone: S64A-36

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1000

Application Note: Anti-TrpC7 Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~100 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TRPC7

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Alternative Name: TrpC7 (TRPC7 Products)

Synonyms: TRP7 antibody, TRP-7 antibody, Trrp8 antibody, TRPC7 antibody, TRPM2 antibody, transient receptor potential cation channel subfamily C member 7 antibody, transient receptor potential cation channel, subfamily C, member 7 antibody, short transient receptor potential channel 7 antibody, TRPC7 antibody, Trpc7 antibody, LOC100012798 antibody

Background:

Synonyms: TRP7, KNP3, TRPM2, transient receptor potential cation channel subfamily C member 7, Transient receptor protein 7

Background: Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a non-selective cation channel that is directly activated by DAG. TrpC7 shows constitutive activity and susceptibility to negative regulartion by extracellular Ca2+. Because of this, TrpC7 plays an important role in the Ca2+ signaling pathway. TrpC7 is also expressed abundantly in the heart, and combined with its ability to act as a Ca2+ channel, TrpC7 might contribute to the process of heart failure.

Gene Name: TRPC7

Gene ID: 57113

NCBI Accession: NP_065122

UniProt: Q9HCX4