NLRP3 antibody (N-Term)

Catalog No. ABIN6657498

Supplier Product No.: 600-401-r14

This Rabbit Polyclonal antibody specifically detects NLRP3 in WB, IHC, IF, FACS and FM. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for NLRP3 antibody (N-Term) (ABIN6657498)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-NLRP3 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-r14

Supplier: Rockland

Purpose: NLRP3 Antibody

Cross-Reactivity (Details): Anti-NLRP3 antibody is directed against human NLRP3 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: NLRP3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of human NLRP3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Flow_Cytometry_Dilution: 1-3 μg/1x106 cells

IF_Microscopy_Dilution: 2 μg/mL

Western_Blot_Dilution: 0.5 μg/mL

Other: ICC: 2 μg/mL

Comment:

Anti-NLRP3 is tested for Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~118.2 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN6657498)

Long, Kou, Li, Wu, Wang: "MiR-223-3p inhibits rTp17-induced inflammasome activation and pyroptosis by targeting NLRP3." in: Journal of cellular and molecular medicine, Vol. 24, Issue 24, pp. 14405-14414, (2021) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3

Background:

Synonyms: Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1,

Background: NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance. This antibody is suitable for researchers interested in cancer research.

Gene Name: NLRP3

Gene ID: 114548

NCBI Accession: NP_001073289

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome