CD11b antibody (PE)

Catalog No. ABIN6657853

Product Details anti-CD11b Antibody (PE)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)

Purification: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b. . Cross-reactivity with CD11b from other sources has not been tested. Anti-CD11b is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Immunogen:

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Clone: M1-70

Isotype: IgG2b

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD11b is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background:

Synonyms: CD11b

Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gene Name: Itgam

UniProt: A1E2I0

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation