SCN1A antibody (C-Term)

Catalog No. ABIN6658122

Product Details anti-SCN1A Antibody

Target: SCN1A (Sodium Channel, Voltage-Gated, Type I, alpha Subunit (SCN1A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCN1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Nav1.1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Nav1.1 from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. No cross reactivity against Nav1.2, Nav1.3 and Nav1.6. Cross-reactivity with Nav1.1 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: Nav1.1 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the cytoplasmic C-terminus region of rat Nav1.1.

Immunogen Type: Recombinant Protein

Clone: S74-71

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Nav1.1 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~220 kDa on specific lysates. Epitope mapped within amino acids 690-715. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SCN1A

Target: SCN1A (Sodium Channel, Voltage-Gated, Type I, alpha Subunit (SCN1A))

Alternative Name: Nav1.1 (SCN1A Products)

Synonyms: SCN9A antibody, SCN1A antibody, B230332M13 antibody, Nav1.1 antibody, EIEE6 antibody, FEB3 antibody, FEB3A antibody, FHM3 antibody, GEFSP2 antibody, HBSCI antibody, NAC1 antibody, SCN1 antibody, SMEI antibody, sodium voltage-gated channel alpha subunit 1 antibody, sodium voltage-gated channel alpha subunit 9 antibody, sodium channel, voltage-gated, type I, alpha antibody, SCN1A antibody, SCN9A antibody, Scn1a antibody

Background:

Synonyms: FEB3, Scn1a, GEFSP2, HBSCI, SCN1, Sodium channel 3

Background: Voltage gated sodium channels initiate action potentials in neurons. Nav1.1 is abundant in the adult brain, and primarily localized in cell bodies. Mutations in the Nav1.1 channels cause generalized epilepsy with febrile seizures plus (GEFS+). Studies show that Nav1.1 channels also play a crucial role in the excitability of cerebellar Purkinje neurons, with major contributions to peak, persistent and resurgent forms of sodium current and to sustained action potential firing.

Gene Name: Scn1a

Gene ID: 20265

NCBI Accession: NP_061203

UniProt: Q62206