KCNA1 antibody (Extracellular Domain)

Catalog No. ABIN6658238

Product Details anti-KCNA1 Antibody

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Binding Specificity: Extracellular Domain

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Anti-Kv1.1 Extracellular Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Kv1.1 from Mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Kv1.1 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: Kv1.1 Extracellular Antibody was produced in mice by repeated immunizations raised against a synthetic peptide at an extracellular domain of rat Kv1.1.

Immunogen Type: Peptide

Clone: S36-15

Isotype: IgG2b

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Kv1.1 Extracellular Antibody is suitable for use in WB and IF microscopy. Expect a band approximately ~56 kDa (could be 65-85 depending on glycosylation) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for KCNA1

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Alternative Name: Kv1.1 (KCNA1 Products)

Synonyms: AEMK antibody, EA1 antibody, HBK1 antibody, HUK1 antibody, KV1.1 antibody, MBK1 antibody, MK1 antibody, RBK1 antibody, KCNA1 antibody, AI840627 antibody, Kca1-1 antibody, Kv1.1 antibody, Mk-1 antibody, Shak antibody, mceph antibody, Kcna antibody, Kcpvd antibody, potassium voltage-gated channel subfamily A member 1 antibody, potassium voltage-gated channel, shaker-related subfamily, member 1 antibody, fragile site, aphidicolin type, common, fra(12)(q24) antibody, KCNA1 antibody, Kcna1 antibody, FRA12E antibody

Background:

Synonyms: KCA1, KCNA1, MBK1, MK1, RBK1, Shak, Voltage-gated potassium channel subunit Kv1.1, Potassium voltage gated channel subfamily A member 1, RBKI, RCK1

Background: Kv1.1, also known as potassium voltage-gated channel subfamily A member 1, is a shaker related voltage potassium channel that in humans is encoded by the SCNA1 gene. It is strongly expressed in a variety of neurons in adult rodents, and it appears to be involved in regulating neuronal excitability. Specifically it plays a role in several developmental processes including proliferation, migration and cell-cell adhesion. The Isaacs syndrome is a result of an autoimmune reaction against the Kv1.1 ion channel.

Gene Name: Kcna1

Gene ID: 24520

NCBI Accession: NP_775118

UniProt: P10499