Leptin antibody (AA 17-100)

Catalog No. ABIN668243

The Rabbit Polyclonal anti-Leptin antibody has been validated for WB, ELISA, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect Leptin in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for Leptin antibody (AA 17-100) (ABIN668243)

Target: Leptin (LEP)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Leptin Antibody

Binding Specificity: AA 17-100

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from bovine Leptin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Leptin antibody (ABIN668243)

Chu, Zhao, Feng, Zhang, Liu, Cheng, Li, Shen, Cao, Li, Min: "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." in: Molecular and cellular endocrinology, Vol. 454, pp. 77-86, (2017) (PubMed).

Zhang, Liu, Li, Ai: "Correlation analysis between the expressions of leptin and its receptor (ObR) and clinicopathology in endometrial cancer." in: Cancer biomarkers : section A of Disease markers, Vol. 14, Issue 5, pp. 353-9, (2015) (PubMed).

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background:

Synonyms: ob, Leptin, Obesity factor, LEP, OBS

Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Gene ID: 280836

UniProt: P50595

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process