Rabbit anti-Mouse IgG2b (Heavy Chain) Antibody (DyLight 488)

Catalog No. ABIN6699070

Supplier Product No.: 610-441-042

Quick Overview for Rabbit anti-Mouse IgG2b (Heavy Chain) Antibody (DyLight 488) (ABIN6699070)

Target: IgG2b

Clonality: Polyclonal

Conjugate: DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 610-441-042

Supplier: Rockland

Purpose: Mouse IgG2b (Gamma 2b chain) Antibody DyLight™ 488 Conjugated

Characteristics: Rabbit Anti-Mouse IgG2b (Gamma 2b chain) Antibody DyLight 488™ Conjugated, Rabbit Anti Mouse IgG2b DyLight 488™ Conjugated Antibody,Anti-Mouse IgG2b DyLight 488 Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain).

Immunogen: Mouse IgG2b heavy chain

Labeling Ratio: 3.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG2b

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.