MED15 antibody (AA 1-285)

Catalog No. ABIN6719342

The Mouse Monoclonal anti-MED15 antibody is suitable to detect MED15 in samples from Human. It has been validated for WB, FACS, IF and ICC.

Quick Overview for MED15 antibody (AA 1-285) (ABIN6719342)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 6F4

Product Details anti-MED15 Antibody

Binding Specificity: AA 1-285

Purpose: Anti-MED15 Antibody (monoclonal, 6F4)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED15 Antibody.(monoclonal, 6F4). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED15 recombinant protein (Position: M1-A285).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background:

Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.

Gene Full Name: mediator complex subunit 15

Molecular Weight: 86 kDa

Gene ID: 51586

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha