RRBP1 antibody

Catalog No. ABIN6719628

Product Details anti-RRBP1 Antibody

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRBP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for RRBP1 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.

Sequence: EKEKKLTSDL GRAATRLQEL LKTTQEQLAR EKDTVKKLQE QLEKAED

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for RRBP1 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence of human RRBP1(EKEKKLTSDLGRAATRLQELLKTTQEQLAREKDTVKKLQEQLEKAED).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RRBP1

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Alternative Name: RRBP1 (RRBP1 Products)

Synonyms: 1700087N07Rik antibody, 5730465C04Rik antibody, ES/130 antibody, mKIAA1398 antibody, mRRp0 antibody, mRRp1.8 antibody, mRRp10 antibody, mRRp15a antibody, mRRp15b antibody, mRRp16.8 antibody, mRRp2 antibody, mRRp41 antibody, mRRp47 antibody, mRRp5.4 antibody, p180 antibody, ES130 antibody, RRp antibody, hES antibody, P180 antibody, rrbp1 antibody, sb:cb489 antibody, wu:fc47a01 antibody, ribosome binding protein 1 antibody, ribosome binding protein 1 homolog 180kDa (dog) antibody, ribosome binding protein 1b antibody, Rrbp1 antibody, RRBP1 antibody, rrbp1b antibody

Background:

Synonyms: Ribosome-binding protein 1, 180 kDa ribosome receptor homolog, RRp, ES/130-related protein, Ribosome receptor protein, RRBP1, KIAA1398

Background: Ribosome-binding protein 1, also referred to as p180, is a protein that in humans is encoded by the RRBP1 gene. It is mapped to 20p12.1. This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12.

Gene ID: 6238

UniProt: Q9P2E9