Vimentin antibody (VIM) (AA 416-466)

Details for Product anti-VIM Antibody No. ABIN672786
Antigen
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 416-466
28
27
22
17
16
16
15
15
15
13
8
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
851
325
309
132
121
80
68
61
55
42
42
40
20
15
12
10
9
6
5
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
418
403
29
12
11
10
10
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
37
24
21
16
14
11
11
11
11
11
11
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
656
341
229
214
193
188
93
63
62
59
40
24
15
12
8
7
7
3
3
3
2
1
1
1
1
1
1
Options
Immunogen KLH conjugated synthetic peptide derived from human Vimentin
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others Vimentin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subcellular location: Cytoplasm

Synonyms: HEL113, CTRCT3, Vimentin, VIM

Gene ID 7431
UniProt P08670
Pathways Caspase Cascade in Apoptosis
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated at ...
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) 20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE an...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Image provided by One World Lab validation program. U138 cells were stained with bs-0...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Image submitted by One World Lab validation program. U138 cells were stained with bs-...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated at 0.2ug f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) This image was kindly submitted by Preethi Vijayaraj from UCLA, 4% PFA fixed cells, p...
Product cited in: Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." in: Journal of tissue engineering and regenerative medicine, 2017 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Human).

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Tanos, Saibene, Pipolo, Battaglia, Felisati, Rubio: "Isolation of putative stem cells present in human adult olfactory mucosa." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181151, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: olfactory mucosa

Park, Sung, Jeong, Choi, Lee, Kwon, Jang, Park, Kim, Kim, Yoon, Ryoo, Kim: "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." in: Cell biochemistry and function, Vol. 35, Issue 7, pp. 392-400, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Sucre, Wilkinson, Vijayaraj, Paul, Dunn, Alva-Ornelas, Gomperts: "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 310, Issue 10, pp. L889-98, 2016 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Human).

Huang, Wen: "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." in: Molecular biology reports, Vol. 43, Issue 10, pp. 1069-78, 2016 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Monteiro, Smith, Angstadt, Zhang, Khademhosseini, Yelick: "Dental cell sheet biomimetic tooth bud model." in: Biomaterials, Vol. 106, pp. 167-79, 2016 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Pig (Porcine)). Further details: Sample: 3D tooth bud constructs

Wilkinson, Alva-Ornelas, Sucre, Vijayaraj, Durra, Richardson, Jonas, Paul, Karumbayaram, Dunn, Gomperts: "Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling." in: Stem cells translational medicine, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: organoids, lung

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Xiao, Li, Qu, Zhang, Pan, Wang, Zhang: "Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer." in: Cellular reprogramming, Vol. 18, Issue 6, pp. 411-418, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF) (Sample species: Cow (Bovine)).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Pan, Li, Duan, Yuan, Fan, Fan, Xiaokaiti, Yang, Wang, Li: "Enoxaparin Sensitizes Human Non-Small-Cell Lung Carcinomas to Gefitinib by Inhibiting DOCK1 Expression, Vimentin Phosphorylation, and Akt Activation." in: Molecular pharmacology, Vol. 87, Issue 3, pp. 378-90, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cheng, Xiang, Yang, Ma, Zhao: "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." in: Cell biochemistry and biophysics, 2015 (PubMed). (Sample species: Rat (Rattus)).

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Fernández-Ferreiro, González Barcia, Gil-Martínez, Vieites-Prado, Lema, Argibay, Blanco Méndez, Lamas, Otero-Espinar: "In vitro and in vivo ocular safety and eye surface permanence determination by direct and Magnetic Resonance Imaging of ion-sensitive hydrogels based on gellan gum and kappa-carrageenan." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 94, pp. 342-51, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Karim, Reese, Wilson, Jacobson, Zhong, Djamali: "Nox2 Is a Mediator of Ischemia Reperfusion Injury." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Li, Inoue, Takamatsu, Kobayashi, Shiraishi, Maeda, Ohashi, Nishida: "Differences between niche cells and limbal stromal cells in maintenance of corneal limbal stem cells." in: Investigative ophthalmology & visual science, Vol. 55, Issue 3, pp. 1453-62, 2014 (PubMed). (Sample species: Human).

Wang, Hu, Gao, Xu, Ning, Zhang: "Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection." in: Journal of virological methods, Vol. 202, pp. 87-94, 2014 (PubMed). (Sample species: Pig (Porcine)).

Sottnik, Campbell, Mehra, Behbahani-Nejad, Hall, Keller: "Osteocytes serve as a progenitor cell of osteosarcoma." in: Journal of cellular biochemistry, Vol. 115, Issue 8, pp. 1420-9, 2014 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: Lung

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