Vimentin antibody (VIM) (AA 416-466)

Details for Product anti-VIM Antibody No. ABIN672786, Supplier: Log in to see
Antigen
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 416-466
28
26
23
22
21
17
16
16
15
13
8
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1021
427
385
259
168
159
147
115
108
76
63
51
47
42
28
27
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
619
366
31
14
12
11
11
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
803
419
369
334
330
275
226
135
86
63
39
26
15
12
8
7
6
3
3
3
3
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen KLH conjugated synthetic peptide derived from human Vimentin
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others Vimentin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subcellular location: Cytoplasm

Synonyms: HEL113, CTRCT3, Vimentin, VIM

Gene ID 7431
UniProt P08670
Pathways Caspase Cascade in Apoptosis
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated at ...
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) 20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE an...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Image provided by One World Lab validation program. U138 cells were stained with bs-0...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Image submitted by One World Lab validation program. U138 cells were stained with bs-...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated at 0.2ug f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Viment...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polycl...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Vimentin (VIM) (AA 416-466) antibody (ABIN672786) This image was kindly submitted by Preethi Vijayaraj from UCLA, 4% PFA fixed cells, p...
Product cited in: Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." in: Journal of tissue engineering and regenerative medicine, 2017 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Human).

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Tanos, Saibene, Pipolo, Battaglia, Felisati, Rubio: "Isolation of putative stem cells present in human adult olfactory mucosa." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181151, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: olfactory mucosa

Park, Sung, Jeong, Choi, Lee, Kwon, Jang, Park, Kim, Kim, Yoon, Ryoo, Kim: "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." in: Cell biochemistry and function, Vol. 35, Issue 7, pp. 392-400, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Sucre, Wilkinson, Vijayaraj, Paul, Dunn, Alva-Ornelas, Gomperts: "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 310, Issue 10, pp. L889-98, 2016 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Human).

Huang, Wen: "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." in: Molecular biology reports, Vol. 43, Issue 10, pp. 1069-78, 2016 (PubMed). Method employed by authors: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Monteiro, Smith, Angstadt, Zhang, Khademhosseini, Yelick: "Dental cell sheet biomimetic tooth bud model." in: Biomaterials, Vol. 106, pp. 167-79, 2016 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Pig (Porcine)). Further details: Sample: 3D tooth bud constructs

Wilkinson, Alva-Ornelas, Sucre, Vijayaraj, Durra, Richardson, Jonas, Paul, Karumbayaram, Dunn, Gomperts: "Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling." in: Stem cells translational medicine, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: organoids, lung

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Xiao, Li, Qu, Zhang, Pan, Wang, Zhang: "Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer." in: Cellular reprogramming, Vol. 18, Issue 6, pp. 411-418, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF) (Sample species: Cow (Bovine)).

Tong, Sun, Wang, Suo: "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." in: Cell biochemistry and biophysics, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Pan, Li, Duan, Yuan, Fan, Fan, Xiaokaiti, Yang, Wang, Li: "Enoxaparin Sensitizes Human Non-Small-Cell Lung Carcinomas to Gefitinib by Inhibiting DOCK1 Expression, Vimentin Phosphorylation, and Akt Activation." in: Molecular pharmacology, Vol. 87, Issue 3, pp. 378-90, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cheng, Xiang, Yang, Ma, Zhao: "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." in: Cell biochemistry and biophysics, 2015 (PubMed). (Sample species: Rat (Rattus)).

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Fernández-Ferreiro, González Barcia, Gil-Martínez, Vieites-Prado, Lema, Argibay, Blanco Méndez, Lamas, Otero-Espinar: "In vitro and in vivo ocular safety and eye surface permanence determination by direct and Magnetic Resonance Imaging of ion-sensitive hydrogels based on gellan gum and kappa-carrageenan." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 94, pp. 342-51, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)).

Karim, Reese, Wilson, Jacobson, Zhong, Djamali: "Nox2 Is a Mediator of Ischemia Reperfusion Injury." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Li, Inoue, Takamatsu, Kobayashi, Shiraishi, Maeda, Ohashi, Nishida: "Differences between niche cells and limbal stromal cells in maintenance of corneal limbal stem cells." in: Investigative ophthalmology & visual science, Vol. 55, Issue 3, pp. 1453-62, 2014 (PubMed). (Sample species: Human).

Wang, Hu, Gao, Xu, Ning, Zhang: "Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection." in: Journal of virological methods, Vol. 202, pp. 87-94, 2014 (PubMed). (Sample species: Pig (Porcine)).

Sottnik, Campbell, Mehra, Behbahani-Nejad, Hall, Keller: "Osteocytes serve as a progenitor cell of osteosarcoma." in: Journal of cellular biochemistry, Vol. 115, Issue 8, pp. 1420-9, 2014 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: Lung

Did you look for something else?