Recombinant Rpb1 CTD antibody (pTyr1)

Catalog No. ABIN6731014

The Mouse Monoclonal anti-Rpb1 CTD antibody is suitable to detect Rpb1 CTD in samples from Human. It has been validated for WB, IF and ELISA.

Quick Overview for Recombinant Rpb1 CTD antibody (pTyr1) (ABIN6731014)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-Rpb1 CTD Antibody

Binding Specificity: pTyr1

Brand: AbFlex®

Purification: Protein A Chromatography

Immunogen: This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Isotype: IgG2a

Application Details

Application Notes:
Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL

For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.

Comment:

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex^® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD

Molecular Weight: 210 kDa