CLIC5 antibody (C-Term)

Catalog No. ABIN6736372

The Rabbit Polyclonal anti-CLIC5 antibody (ABIN6736372) specifically detects CLIC5 in WB, IHC and IHC (p). The antibody is reactive with Human, Guinea Pig, Horse, Rabbit, Cow, Pig, Monkey and Bat samples.

Quick Overview for CLIC5 antibody (C-Term) (ABIN6736372)

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Reactivity: Human, Guinea Pig, Horse, Rabbit, Cow, Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CLIC5 Antibody

Binding Specificity: C-Term

Specificity: Human CLIC5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Mouse, Rat, Dog, Xenopus (92%) Chicken (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CLIC5 (Q53G01, NP_058625). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Mouse, Rat, Elephant, Dog, Opossum, Turkey, Zebra finch, Sparrow, Platypus, Lizard, Xenopus, Salmon, Zebrafish (92%), Chicken (91%), Stickleback (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CLIC5

Target: CLIC5 (Chloride Intracellular Channel 5 (CLIC5))

Alternative Name: CLIC5

Background: Name/Gene ID: CLIC5
Subfamily: Chloride channel - intracellular
Family: Ion Channel

Synonyms: CLIC5, CLIC5B, MST130, MSTP130

Gene ID: 53405

NCBI Accession: NP_058625

UniProt: Q9NZA1

Pathways: Sensory Perception of Sound