FOXA3 antibody (AA 201-250)

Catalog No. ABIN214752

This anti-FOXA3 antibody is a Rabbit Polyclonal antibody detecting FOXA3 in WB, IHC and IHC (p). Suitable for Human, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Pig and Monkey.

Quick Overview for FOXA3 antibody (AA 201-250) (ABIN214752)

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Reactivity: Human, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXA3 Antibody

Binding Specificity: AA 201-250

Brand: IHC-plus™

Specificity: Human FOXA3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Mouse, Rat, Horse (92%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa201-250 of human FOXA3 (P55318, NP_004488). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Pig, Opossum, Guinea pig (100%), Mouse, Rat, Horse (92%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXA3

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Alternative Name: FOXA3

Background: Name/Gene ID: FOXA3
Family: Transcription factor

Synonyms: FOXA3, Forkhead box A3, HNF-3G, HNF3G, HNF-3-gamma, Forkhead box protein A3, TCF3G, TCF-3G, FKHH3, Transcription factor 3G

Gene ID: 3171

NCBI Accession: NP_004488

UniProt: P55318

Pathways: Carbohydrate Homeostasis