GABRE antibody (AA 237-286)

Catalog No. ABIN6736386

The Rabbit Polyclonal anti-GABRE antibody has been validated for WB, IHC (p) and IHC. It is suitable to detect GABRE in samples from Human, Mouse, Guinea Pig, Rabbit and Monkey.

Quick Overview for GABRE antibody (AA 237-286) (ABIN6736386)

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Reactivity: Human, Mouse, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-GABRE Antibody

Binding Specificity: AA 237-286

Brand: IHC-plus™

Specificity: Human GABRE

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat (92%) Rabbit (85%) Mouse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa237-286 of human GABRE. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Rat (92%), Rabbit (85%), Mouse (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GABRE

Target: GABRE (gamma-aminobutyric Acid (GABA) A Receptor, epsilon (GABRE))

Alternative Name: GABRE

Background: Name/Gene ID: GABRE
Subfamily: GABA A receptor
Family: Ion Channel

Synonyms: GABRE, GABA receptor epsilon, GABA(A) receptor, epsilon

Gene ID: 2564

UniProt: P78334

Pathways: Synaptic Membrane