His Tag antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN6737086

This Mouse Monoclonal antibody specifically detects His Tag in WB and ELISA.

Quick Overview for His Tag antibody (Alkaline Phosphatase (AP)) (ABIN6737086)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody (Alkaline Phosphatase (AP))

Sequence: HHHHHH

Specificity: Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Purification: Affinity purified

Immunogen: His-tag-fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. ELISA: 1:10000-1:50000. Use 50-100 ng control antigen/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. APC is light sensitive.

Target Details for His Tag

Target: His Tag

Target Type: Tag