His Tag antibody (HRP)

Catalog No. ABIN6737087

The Mouse Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, ELISA and IHC.

Quick Overview for His Tag antibody (HRP) (ABIN6737087)

Target: His Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-His Tag Antibody (HRP)

Sequence: HHHHHH

Specificity: Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Purification: Immunoaffinity purified

Immunogen: His-tag fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC (1:200 - 1:2000), WB (1:10000)

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Western Blot: 1:10000. ELISA: 1:10000-1:50000 using 50-100 ng control antigen/well. Immunohistochemistry: 1:200-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 % merthiolate

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Target Details for His Tag

Target: His Tag

Target Type: Tag