RASSF1 antibody (AA 145-260)

Catalog No. ABIN673764

This anti-RASSF1 antibody is a Rabbit Polyclonal antibody detecting RASSF1 in ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for RASSF1 antibody (AA 145-260) (ABIN673764)

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASSF1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RASSF1 Antibody

Binding Specificity: AA 145-260

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RASSF1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-RASSF1 antibody (ABIN673764)

Klacz, Wierzbicki, Wronska, Rybarczyk, Stanislawowski, Slebioda, Olejniczak, Matuszewski, Kmiec: "Decreased expression of RASSF1A tumor suppressor gene is associated with worse prognosis in clear cell renal cell carcinoma." in: International journal of oncology, Vol. 48, Issue 1, pp. 55-66, (2015) (PubMed).

Liu, Ao, Zhou, Cui, Zhou, Yuan, Xiang, Cao, Liu: "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." in: Biochemical and biophysical research communications, Vol. 402, Issue 3, pp. 507-14, (2010) (PubMed).

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: RASSF1

Background:

Synonyms: 123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association domain-containing protein 1, RASSF1

Background: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF1 stimulation. Isoform A interacts with CDC2, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

Gene ID: 11186

UniProt: Q9NS23