TLE2 antibody (AA 201-250)

Catalog No. ABIN6737675

Product Details anti-TLE2 Antibody

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Binding Specificity: AA 201-250

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human TLE2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa201-250 of human TLE2 (Q04725, NP_003251). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Elephant, Dog, Bovine, Horse (100%), Mouse, Rat, Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2 (TLE2 Products)

Synonyms: TLE2 antibody, ESG antibody, ESG2 antibody, GRG2 antibody, Grg2 antibody, mKIAA4188 antibody, transducin like enhancer of split 2 antibody, transducin-like enhancer of split 2 antibody, TLE2 antibody, Tle2 antibody

Background: Name/Gene ID: TLE2

Synonyms: TLE2, ESG, Enhancer of split groucho 2, ESG2, GRG2

Gene ID: 7089

NCBI Accession: NP_003251

UniProt: Q04725

Pathways: WNT Signaling