BDNF antibody (AA 146-195)

Catalog No. ABIN6738014

The Rabbit Polyclonal anti-BDNF antibody has been validated for WB. It is suitable to detect BDNF in samples from Human, Rat, Mouse, Pig, Horse, Dog, Guinea Pig, Cow, Hamster, Monkey and Xenopus laevis.

Quick Overview for BDNF antibody (AA 146-195) (ABIN6738014)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse, Pig, Horse, Dog, Guinea Pig, Cow, Hamster, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BDNF Antibody

Binding Specificity: AA 146-195

Specificity: Human BDNF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human BDNF (P23560, NP_001700). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Hamster, Dolphin, Elephant, Panda, Dog, Cat, Bovine, Horse, Pig, Guinea pig, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Gene ID: 627

NCBI Accession: NP_001700

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity