PAI1 antibody (AA 31-80)

Catalog No. ABIN6738364

The Rabbit Polyclonal anti-PAI1 antibody has been validated for WB. It is suitable to detect PAI1 in samples from Human, Mouse, Rat, Rabbit, Pig, Cow and Monkey.

Quick Overview for PAI1 antibody (AA 31-80) (ABIN6738364)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAI1 Antibody

Binding Specificity: AA 31-80

Specificity: Human SERPINE1 / PAI-1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa31-80 of human SERPINE1 (P05121, NP_000593). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Panda, Bovine, Rabbit, Pig (100%), Galago, Elephant, Dog (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1 / PAI-1

Background: Name/Gene ID: SERPINE1
Family: Serine Protease Inhibitor

Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1

Gene ID: 5054

NCBI Accession: NP_000593

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration