PAI1 antibody (C-Term)

Catalog No. ABIN6738365

This anti-PAI1 antibody is a Rabbit Polyclonal antibody detecting PAI1 in WB. Suitable for Human, Mouse, Rat, Rabbit, Pig, Dog, Cow, Guinea Pig, Monkey and Bat.

Quick Overview for PAI1 antibody (C-Term) (ABIN6738365)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Dog, Cow, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAI1 Antibody

Binding Specificity: C-Term

Specificity: Human SERPINE1 / PAI-1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit (100%) Xenopus (92%) Dog (87%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SERPINE1 (P05121, NP_000593). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Bovine, Bat, Rabbit, Pig, Guinea pig (100%), Opossum, Platypus, Xenopus (92%), Dog (87%), Mouse, Rat, Salmon (85%), Goat, Horse (84%), Zebrafish, Drosophila, Mosquito (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1 / PAI-1

Background: Name/Gene ID: SERPINE1
Family: Serine Protease Inhibitor

Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1

Gene ID: 5054

NCBI Accession: NP_000593

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration