MAP3K1 antibody (AA 1251-1300)

Catalog No. ABIN6738489

This Rabbit Polyclonal antibody specifically detects MAP3K1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Rabbit, Pig, Cow, Guinea Pig, Horse, Dog, Bat and Chicken.

Quick Overview for MAP3K1 antibody (AA 1251-1300) (ABIN6738489)

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Pig, Cow, Guinea Pig, Horse, Dog, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP3K1 Antibody

Binding Specificity: AA 1251-1300

Specificity: Human MAP3K1 / MEKK1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1251-1300 of human MAP3K1 (Q13233, NP_005912). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Lizard (100%), Platypus (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP3K1

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Alternative Name: MAP3K1 / MEKK1

Background: Name/Gene ID: MAP3K1
Subfamily: MAP3K
Family: Protein Kinase

Synonyms: MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MAP/ERK kinase kinase 1, MEK kinase 1, MEKK, MEKK 1, MEKK1, SRXY6

Gene ID: 4214

NCBI Accession: NP_005912

UniProt: Q13233

Pathways: MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades