ARAF antibody (AA 254-303)

Catalog No. ABIN6738961

The Rabbit Polyclonal anti-ARAF antibody has been validated for WB. It is suitable to detect ARAF in samples from Human, Mouse, Rat, Cow, Pig, Sheep, Horse and Bat.

Quick Overview for ARAF antibody (AA 254-303) (ABIN6738961)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human, Mouse, Rat, Cow, Pig, Sheep, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARAF Antibody

Binding Specificity: AA 254-303

Specificity: Human ARAF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Horse, Pig (100%) Dog, Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human ARAF (P10398, NP_001645). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Bovine, Bat, Horse, Pig (100%), Marmoset, Dog, Rabbit (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF / ARAF1 / A-RAF

Background: Name/Gene ID: ARAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: ARAF, A RAF, A-RAF, A-raf-1, PKS, PKS2, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Ras-binding protein DA-Raf, Oncogene ARAF1, ARAF1, Proto-oncogene Pks, RAFA1

Gene ID: 369

NCBI Accession: NP_001645

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C