Insulin Receptor antibody (AA 863-912)

Catalog No. ABIN6741677

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey, Horse, Guinea Pig, Pig and Bat.

Quick Overview for Insulin Receptor antibody (AA 863-912) (ABIN6741677)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Guinea Pig, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: AA 863-912

Specificity: Human INSR / Insulin Receptor

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig (100%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa863-912 of human INSR (P06213, NP_000199). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig (100%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR / Insulin Receptor

Background: Name/Gene ID: INSR
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: INSR, CD220, ILP receptor, Insulin receptor, IR, HHF5, CD220 antigen

Gene ID: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity