PAI1 antibody (AA 251-300)

Catalog No. ABIN6741811

The Rabbit Polyclonal anti-PAI1 antibody has been validated for WB. It is suitable to detect PAI1 in samples from Human, Mouse, Rat, Rabbit, Pig, Cow, Horse, Goat and Zebrafish (Danio rerio).

Quick Overview for PAI1 antibody (AA 251-300) (ABIN6741811)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow, Horse, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAI1 Antibody

Binding Specificity: AA 251-300

Specificity: Human SERPINE2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig, Xenopus (92%) Bovine, Goat, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human SERPINE2 (P07093, NP_006207). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Goat, Rabbit, Horse, Pig, Opossum (100%), Marmoset, Dog, Bat, Guinea pig, Platypus, Lizard, Xenopus (92%), Elephant, Bovine, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE2 / Nexin

Background: Name/Gene ID: SERPINE2

Synonyms: SERPINE2, GDNPF, GDN, PNI, PN1, Protease nexin I, Nexin, Protease nexin 1, Serpin E2, Glia-derived nexin, Peptidase inhibitor 7, PI-7, PI7, PN-1

Gene ID: 5270

NCBI Accession: NP_006207

UniProt: P07093

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration