Estrogen Receptor alpha antibody (AA 35-84)

Catalog No. ABIN6741863

This anti-Estrogen Receptor alpha antibody is a Rabbit Polyclonal antibody detecting Estrogen Receptor alpha in WB. Suitable for Human, Mouse, Rabbit, Monkey and Horse.

Quick Overview for Estrogen Receptor alpha antibody (AA 35-84) (ABIN6741863)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rabbit, Monkey, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 35-84

Specificity: Human ESR1 / Estrogen receptor

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat, Bovine, Pig (92%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human ESR1 (P03372, NP_000116). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Tamarin, Rabbit (100%), Baboon, Rat, Cat, Bovine, Bat, Pig, Guinea pig (92%), Mouse, Hamster (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ER Alpha / Estrogen Receptor

Background: Name/Gene ID: ESR1
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha

Gene ID: 2099

NCBI Accession: NP_000116

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly